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dulbecco s phosphate buffered saline dpbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Dulbecco S Phosphate Buffered Saline Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dulbecco+s+phosphate/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula/pmc13241941-84-15-24
    Average 99 stars, based on 1 article reviews
    dulbecco s phosphate buffered saline dpbs - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Tooth Decay Promotes Senescence in Dental Pulp Stem Cells, Modifying Their Biological and Proteomic Profiles
    Article Snippet: Samples were digested via collagenase (Type II, 125 U/ml) (Sigma‐Aldrich, St. Louis, MO, USA) for approximately 45 min at 37°C thermal block. .. Cells were washed twice with Dulbecco's Phosphate‐Buffered Saline (DPBS) (Ca, Mg‐free) (Gibco, Waltham, MA, USA) after the digestion process. .. Cell pellet was resuspended at 10% Fetal Bovine Serum (FBS) (FBS; Gibco, Waltham, MA, USA), 25 ng/mL l ‐Ascorbic Acid (Sigma‐Aldrich, St. Louis, MO, USA), 4 ng/mL dexamethasone (Sigma‐Aldrich, St. Louis, MO, USA), 5 ng/mL basic Fibroblast Growth Factor (b‐FGF) (Gibco, Waltham, MA, USA), and 10 ng/mL Epidermal Growth Factor (EGF) (Gibco, Waltham, MA, USA) containing Alpha Minimum Essential Medium (α‐MEM) (with nucleosides) (Gibco, Waltham, MA, USA) and was cultured at 37°C.

    Article Title: Human induced pluripotent stem cell‐derived mesenchymal stromal cells regenerate diabetic ischemic muscle
    Article Snippet: To induce MSC differentiation from hiPSC, we used the STEMdiffTM Mesenchymal Progenitor Kit (Stemcell Technologies, Vancouver, BC, Canada) according to the manufacturer's protocol. .. Briefly, hiPSCs were rinsed with Dulbecco's phosphate‐buffered saline (DPBS) and then treated with 2 mL of Accutase (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) for 3–5 min. Next, 8 mL of mTeSRTM1 media was added. ..

    Article Title: Lifelong Evolution of Autoreactive Plasma Cell Numbers, Affinity, and Anatomic Location in Arthritic K/ BxN Mice
    Article Snippet: .. Streptavidin‐coated paramagnetic nanobeads (03231; Ademtech) were incubated in Dulbecco's phosphate‐buffered saline (DPBS) without calcium and magnesium (Gibco) containing 1 μM CaptureSelect biotin anti‐κ light chain conjugate (Thermo Fisher Scientific) for 20 minutes at room temperature and washed and incubated in 5% Pluronic F68 solution (P6866; Thermo Fisher Scientific) for 20 minutes at room temperature. .. Nanobeads were incubated in DropMap buffer for 20 minutes at room temperature, washed in DPBS and resuspended in DropMap buffer containing 60 nM of AF488‐labeled GPI (final in‐droplet concentration 30 nM) and 150 nM of an AF647‐labeled rabbit anti‐mouse IgG F(ab’) 2 (final in‐droplet concentration: 75 nM; 315‐606‐046; Jackson ImmunoResearch).

    Article Title: Structure-Guided Engineering of 3D8 scFv Enhances Stability and Antiviral Potential.
    Article Snippet: .. After incubation, the medium was removed, and each well was washed with 1 mL of 1× Dulbecco's phosphate‐buffered saline (DPBS; Gibco, USA). ..

    Article Title: Tooth Decay Promotes Senescence in Dental Pulp Stem Cells, Modifying Their Biological and Proteomic Profiles.
    Article Snippet: Samples were digested via collagenase (Type II, 125 U/ml) (Sigma‐Aldrich, St. Louis, MO, USA) for approximately 45 min at 37°C thermal block. .. Cells were washed twice with Dulbecco's Phosphate‐Buffered Saline (DPBS) (Ca, Mg‐free) (Gibco, Waltham, MA, USA) after the digestion process. .. Cell pellet was resuspended at 10% Fetal Bovine Serum (FBS) (FBS; Gibco, Waltham, MA, USA), 25 ng/mL L‐Ascorbic Acid (Sigma‐Aldrich, St. Louis, MO, USA), 4 ng/mL dexamethasone (Sigma‐Aldrich, St. Louis, MO, USA), 5 ng/mL basic Fibroblast Growth Factor (b‐FGF) (Gibco, Waltham, MA, USA), and 10 ng/mL Epidermal Growth Factor (EGF) (Gibco, Waltham, MA, USA) containing Alpha Minimum Essential Medium (α‐MEM) (with nucleosides) (Gibco, Waltham, MA, USA) and was cultured at 37°C.

    Article Title: Filament Extrusion‐Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro
    Article Snippet: .. Cell culture reagents, including Fetal Bovine Serum (FBS), trypsin–EDTA, Dulbecco's Modified Eagle Medium (DMEM), and Dulbecco's Phosphate‐Buffered Saline (DPBS), were sourced from Gibco (New York, NY, USA). .. The LIVE/DEAD Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing
    Article Snippet: .. For passaging, the INS1 β‐cells were washed with 1×Dulbecco's phosphate‐buffered saline (DPBS, VWR, Radnor, PA, USA) before incubation with trypsin‐EDTA (Gibco, ThermoFisher Scientific) for 3 min and resuspension in complete medium. ..

    Article Title: Type III Collagen Promotes a Pro-Fibrotic Microenvironment for In Vitro Tendon Healing Microphysiological Systems.
    Article Snippet: Microphysiological systems of tendon healing, or Tendon‐on‐Chips, are promising platforms for studying scarring and fibrotic repair and can be relevant for the development of novel anti‐fibrotic therapeutics.. Effective fibrosis models must accurately recreate the myofibroblast microenvironment of signals that modulate their activation and drive peritendinous adhesions.. These include not only the cellular interactions between resident tendon cells and proliferating immune and endothelial cells, but importantly, the dynamic extracellular matrix changes.

    Incubation:

    Article Title: Lifelong Evolution of Autoreactive Plasma Cell Numbers, Affinity, and Anatomic Location in Arthritic K/ BxN Mice
    Article Snippet: .. Streptavidin‐coated paramagnetic nanobeads (03231; Ademtech) were incubated in Dulbecco's phosphate‐buffered saline (DPBS) without calcium and magnesium (Gibco) containing 1 μM CaptureSelect biotin anti‐κ light chain conjugate (Thermo Fisher Scientific) for 20 minutes at room temperature and washed and incubated in 5% Pluronic F68 solution (P6866; Thermo Fisher Scientific) for 20 minutes at room temperature. .. Nanobeads were incubated in DropMap buffer for 20 minutes at room temperature, washed in DPBS and resuspended in DropMap buffer containing 60 nM of AF488‐labeled GPI (final in‐droplet concentration 30 nM) and 150 nM of an AF647‐labeled rabbit anti‐mouse IgG F(ab’) 2 (final in‐droplet concentration: 75 nM; 315‐606‐046; Jackson ImmunoResearch).

    Article Title: Structure-Guided Engineering of 3D8 scFv Enhances Stability and Antiviral Potential.
    Article Snippet: .. After incubation, the medium was removed, and each well was washed with 1 mL of 1× Dulbecco's phosphate‐buffered saline (DPBS; Gibco, USA). ..

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing
    Article Snippet: .. For passaging, the INS1 β‐cells were washed with 1×Dulbecco's phosphate‐buffered saline (DPBS, VWR, Radnor, PA, USA) before incubation with trypsin‐EDTA (Gibco, ThermoFisher Scientific) for 3 min and resuspension in complete medium. ..

    Cell Culture:

    Article Title: Filament Extrusion‐Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro
    Article Snippet: .. Cell culture reagents, including Fetal Bovine Serum (FBS), trypsin–EDTA, Dulbecco's Modified Eagle Medium (DMEM), and Dulbecco's Phosphate‐Buffered Saline (DPBS), were sourced from Gibco (New York, NY, USA). .. The LIVE/DEAD Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Modification:

    Article Title: Filament Extrusion‐Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro
    Article Snippet: .. Cell culture reagents, including Fetal Bovine Serum (FBS), trypsin–EDTA, Dulbecco's Modified Eagle Medium (DMEM), and Dulbecco's Phosphate‐Buffered Saline (DPBS), were sourced from Gibco (New York, NY, USA). .. The LIVE/DEAD Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Passaging:

    Article Title: Nanopore Electroporation: A New Delivery Method Within the Field of Epigenetic Editing
    Article Snippet: .. For passaging, the INS1 β‐cells were washed with 1×Dulbecco's phosphate‐buffered saline (DPBS, VWR, Radnor, PA, USA) before incubation with trypsin‐EDTA (Gibco, ThermoFisher Scientific) for 3 min and resuspension in complete medium. ..



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